Supplementary Figure 14: T-SNE Visualization Of Exposed And Unexposed Macrophages Using A 5,000 Transcript Cutoff
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
(a) Using a threshold of 5,000 detected transcripts, we identified 4,638 macrophages. (b) Among these 4,638 cells, we identified 5 distinct clusters of macrophages by performing graph-based clustering over 5 principal components (377 variable genes). (c) Clusters 1-3 are defined by unique gene expression signatures, while Clusters 4 and 5 are defined by expression of mitochondrial genes, suggesting low-quality cells. (d) Following removal of cells within Clusters 4 and 5, there remain a total of 2,560 cells in Clusters 1-3.
