Supplementary Figure 3: Bead And Cell Loading Efficiency
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
(a) Two arrays were loaded with barcoded beads through intermittent rocking. After washing, arrays were imaged in transmitted light and AF488 channel to capture bead autofluorescence. A plot of the frequency of the 75th percentile AF488 well intensity across the array (Panel 1) and the frequency of wells containing zero, one and multiple beads is displayed (Panel 2). (b) 200 μL of a 1:1 mix of fluorescently labeled human (HEK 293) and mouse (3T3) cell solution was loaded into 3 arrays and 12 wells of a 96 well plate. The number of cells loaded into each array and well as enumerated by fluorescent imaging is plotted, normalized to the average number of cells/well in the 96 well plate. Mean and standard error are denoted by line and error bars respectively. (c) 2x102, 2x103, and 2x104 total cells of a 1:1 mixture of fluorescently labeled HEK 293T and 3T3 cells were loaded onto three functionalized arrays each. All arrays were fluorescently imaged to enumerate the number of each cell line in each array microwell. The mean ± standard deviation of the number of empty, single and multiple occupancy wells across the three replicate arrays for each loading density is displayed along with the mean ± standard deviation of the percentage of occupied wells containing a cell from each species
