Supplementary Figure 5: Microwell Sealing With Semipermeable Membrane
From: Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
PBMCs labeled with αCD45-AF647 were loaded into two BSA-blocked arrays and one array functionalized with chitosan and poly(glutamate). A semipermeable membrane was attached to one of the BSA-blocked arrays and the chitosan:polyglutamate functionalized array prior to addition of lysis buffer. (a) Example images of transmitted light and AF647 fluorescence of the arrays before, and 5 and 30 minutes after addition of lysis buffer are displayed for each array. (b) The total fluorescence intensity (FI) of all pixels associated with cells within a well is plotted against the median fluorescent intensity (MFI) of the volume of the same well 5 minutes after lysis for 12,100 wells from each array. (c) The MFI of the well volume 5 minutes after lysis is plotted against the MFI of the volume of the same well 30 minutes after lysis for the same 12,100 wells from each array.
