Supplementary Figure 11: GUI for correction of marker onset annotations.
From: Prospective identification of hematopoietic lineage choice by deep learning
(a) Genealogy overview. Annotations can be inspected in every branch (black: unknown, blue: GM, red: MegE) (b) Annotation view. Dropdown menus allow to select different experiments, genealogies and branches from the dataset. After selection, the quantifications for every cell in the branch are loaded. Cell size (first panel) is used to check cell identification quality and to calculate fluorescence concentrations. PU.1-eYFP concentration (second panel) is not used for annotation, whereas GATA1-mCherry (third panel) and CD16/32 antibody concentration (fourth panel) can be used to change the annotation timepoint of a full branch by clicking on the respective frame (red line). The identified single cell in brightfield (upper right image) and its fluorescence signal for the changed channel (lower right image) are shown for visual quality control. Note that high levels of GATA1-mCherry signal at movie start stem from the antibody staining used for HSPC purification by FACS.
