Supplementary Figure 1: Experimental setup.
From: Prospective identification of hematopoietic lineage choice by deep learning
Primary murine bone marrow cells are extracted from the bone marrow of adult mice. Hematopoietic stem and progenitor cells (HSPCs) are purified by fluorescence activated cell sorting (FACS) and cultivated on plastic with added CD16/32 antibody. Cells are imaged in brightfield and three fluorescence channels for up to 8 days. After the experiment is stopped, single HSPCs and their progeny are manually tracked and annotated.
