Supplementary Figure 5: Unknown and latent cells predicted to be either GM or MegE committed express significantly different PU.1-eYFP levels.
From: Prospective identification of hematopoietic lineage choice by deep learning
Same analysis as described in Figure 2, but for (a) train-test round 2 and (b) train-test round 3. (c) Amount of unknown or latent (white boxes) versus annotated cells (blue and red boxes), summed over all experiments. 2±1% (mean±sd, n=3 rounds) of GM and 15±8% of MegE marker onsets were annotated earlier than four generations after experiment start. Our approach is able to prospectively detect lineage commitment from generation 3 onwards (black arrow), outperforming the conventional manual marker-based approach.
