Supplementary Figure 5: Revisit of the same population of neurons in the SP layer of the CA1 region of the hippocampus after one week.
From: In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
a, Activity recording site in the SP layer of the hippocampus located at 984 μm beneath the dura (left). The neuronal population was the same as that shown in Figure 2(c) and Supplementary Figure 2, but imaged one week later. Neurons are indexed (right) for reference to their traces in (b) and Supplementary Figure 6. Average power of 58 mW at 800 kHz was used for imaging. The FOV was 150x150 μm. Scale bar 20 μm.
b, Spontaneous activity traces recorded from three indexed neurons in (a) for 10 minutes, at a frame rate of 8.49 Hz. The three neurons represent a range of activity level and brightness. To the left of each trace is the index of the neuron. All traces were low-pass filtered with a hamming window with a 0.59 s time constant, and fluorescent intensity was converted to photon counts per neuron per second.
