Supplementary Figure 1: A doming model describes the motion of frozen hydrated samples induced by the high-energy electron beam.

(a) Traces of the projected motion measured at three different tilt angles (α-angle) extracted from a dose fractionated tilt series acquired on a frozen hydrated specimen of a wild-type Drosophila melanogaster γ-Tubulin Ring Complex. The black arrow in the plot indicates the starting position of the motion. (b) Image of frozen hydrated archaeal 20S proteasome overlaid with the traces of global motion based upon whole frame alignment (long trace originated from the center of image) and each patch determined from MotionCor2. The whole frame is divided into 5 × 5 patches, and traces of each patch are determined individually. The traces of the global and local motions are exaggerated on the image by a factor of 145. For perspective, the accumulated global motion is ∼11 A.