Supplementary Figure 4: Consistency of estimates on SEQC data within and between centers
From: Salmon provides fast and bias-aware quantification of transcript expression
The distribution of the mean absolute error of (inverse hyperbolic sine-transformed) TPMs between different replicates of data from the SEQC [12] study. The A sample corresponds to universal human reference tissue (UHRR) and the B sample corresponds to human brain tissue (HBRR). When comparing the replicates that were sequenced at different centers, the inter-replicate distances are larger. However, we observe that Salmon’s bias correction methodology results in improved consistency (i.e., reduced distances) compared to the estimates produced by other methods, especially when comparing replicates sequenced at different centers, where we expect the effects of bias to be more pronounced.
