Supplementary Figure 1: Comparison and overview of spike-in sets.
From: Power analysis of single-cell RNA-sequencing experiments
ERCC spike-ins consist of 92 very distinct sequences based on bacterial genes logarithmically distributed across 22 abundance levels (in Mix 1), with poly-A tails ranging from 20 to 26 base pairs. SIRV spike-ins are 69 sequences, modeled after sequences and splicing patterns in 7 human genes. In Mix 2, which we used, the SIRV molecules are present at 4 abundance levels, with virtual alternative isoforms from each gene present at each abundance level. All SIRV molecules have 30 base pair long poly-A tails.
