Supplementary Figure 5: Validation of the pcSOFi normalization algorithm.
From: Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
(A) and (C) show average fluorescence images of plasma membrane targeted FLINC-AKAR1, where uneven distribution of the probe could be observed. (B) and (D) show normalized pcSOFI images of the same cells. (E) The effectiveness of the pcSOFI normalization scheme is demonstrated by performing normalization on HeLa cells expressing membrane targeted Dronpa. Normalization removes the clearly present concentration bias, as seen by the profile (averaged along the y-axis of the region indicated by the red box). All scalebars are 10 ÎĽm.
