Supplementary Figure 6: Additional control data supporting the accuracy, biological relevance and function of FLINC biosensing.
From: Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
(A) Treatment of Fsk/IBMX stimulated HeLa cells with 20 ÎĽM H89 over time can inhibit PKA activity to baseline levels. (B) FLINC-AKAR1 is sensitive to intermediate levels of PKA activity as demonstrated by representative response during 1) H89 inhibition (left column of B), and 2) submaximal forskolin (Fsk) dosage (right column of B). The response of the FRET-based biosensor is provided for comparison in each case. In (A), center line and whiskers mark mean and s.e.m. values, respectively.
