Supplementary Figure 12: Detection of the weak protein–protein interaction between FHA1 and p-PKAsub.
From: Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
(A) Schematic of the bi-molecular version of FLINC-AKAR1. It responses to PKA stimulation at the membrane by detecting the binding of phospho-substrate and FHA1, a weak protein-protein interaction (kD ~ 0.5 ÎĽM). (B) 11 min after Fsk/IBMX stimulation the wild-type (WT, n=3) biosensor shows significant response compare to the non-phosphorylatable mutant (TA, n=4). Pair-wise t-test result *: p<0.05; center line and whiskers mark mean and s.e.m. values, respectively.
