Supplementary Figure 3: The characteristic photophysical differences between DpTT and TT (TagRFP-T) are primarily observed in dark-state conversion.
From: Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
(A) A simplified schematic depicting the 4-state model of the TagRFP-T chromophore transition. (B) Results of the Dark-State Conversion (DSC) measurement and fit. The bottom two figures summarize the difference in DSC time constant and Percent DSC. (C) Results of the Ground-State Recovery (GSR) measurement and fit. (D) Results of the fluorescence (em) life time measurement; IRF is instrument response function. Pair-wise t-test results in (B) are marked where data were compared with the construct marked “ref”; n numbers are marked in the corresponding columns. *: p<0.05. Center line and whiskers mark mean and s.e.m. values, respectively.
