Supplementary Figure 2: Segmental labeling of EMCVDElinkF-RNA. | Nature Methods

Supplementary Figure 2: Segmental labeling of EMCVDElinkF-RNA.

From: Structural modeling of protein–RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS

Supplementary Figure 2

a, strategy for segmental labeling as employed in our study. Notably, only one aliquot of isotope labeled RNA is used independent of the size of the RNA and the number of isotope labeled segments. b, RNAse H digestion of EMCVDElinkF with chimSLD (lane 1), chimLinkF (lane 2), chimSLE (lane 3) and all three chimeras simultaneously (lane 5). The full-length RNA is shown in lane 4, an example for splinted RNA-ligation is shown in lane 6 (input) and 7 (ligation). Note that the isotope labeled RNA fragment is kept as limiting substrate in the reaction.

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