Supplementary Figure 4: RRM2 binds preferentially to EMCVF. | Nature Methods

Supplementary Figure 4: RRM2 binds preferentially to EMCVF.

From: Structural modeling of protein–RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS

Supplementary Figure 4

a, Upon titration of RRM12 with EMCVF only the RRM2 moiety reaches saturation at a 1:1 ratio (EMCVF:RRM12) and signals overlap almost perfectly with signals of the RRM2-EMCVF complex (yellow). b, In the presence of SLE, RRM1 and RRM2 bind SLE and SLF, respectively, as indicated by the almost perfect superimposition of spectra collected on RRM1-EMCVE (green), RRM2-EMCVF (yellow) and RRM12-EMCVEmutF (blue; right) complexes.

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