Supplementary Figure 7: Cell sorting and genotyping procedures for patients
(a) Contour plots describing the sorting strategy for isolating HSCs in patient 2 (the same was done for patient 1). CD34, CD38, CD90 and CD45RA expression is displayed using a log scale; (b) Lineage negative, CD34+/CD38-/CD90+/CD45RA- single cells were sorted into individual wells for scRNA-Seq or colony growth in cytokine cocktail allowing progenitor cell expansion. For genotyping the JAK2V617F and the TET2 loci were characterised using Sanger sequencing. (c) Clonal composition of patients 1, 2 obtained by Sanger sequencing experiments as described in (b) of the JAK2V617F and the TET2 loci (Methods). Colors are the same as Cluster colors in Fig. 3.
