Supplementary Figure 12: Additional lines of evidence that SC3 can help to define subclonal composition | Nature Methods

Supplementary Figure 12: Additional lines of evidence that SC3 can help to define subclonal composition

From: SC3: consensus clustering of single-cell RNA-seq data

Supplementary Figure 12

(a) Comparison of the coefficient of variation of gene expression in Tet2 and WT subclones of patient 1; (b) Sorting of haematopoietic stem and progenitor cells from patient 1 and 2 using antibodies that target surface markers identified using SC3. Our analysis suggests that CD83 should be specific for WT clones, CD127 and CD244 for the Tet2 only mutant clones, while CD82 is specific to double mutant clones. Percentages account for CD38+CD34+ cells positive for the indicated surface marker.

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