Supplementary Figure 7: Generation of T cells from multiple HSPC sources and subsets. | Nature Methods

Supplementary Figure 7: Generation of T cells from multiple HSPC sources and subsets.

From: Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids

Supplementary Figure 7

(a) Persistence of CD34+ cells in week 6 ATOs initiated with human cord blood (CB), adult bone marrow (BM), G-CSF mobilized peripheral blood (MPB), or non-mobilized peripheral blood (PB) HSPCs. (b) T cell progenitor subsets in ATOs from different HSPC sources, gated on CD34+ cells as shown in (a). (c) CD34+ progenitors and (d) CD34+ progenitor subsets in week 6 ATOs initiated with hematopoietic stem cell (HSC)-enriched (Lin-CD34+CD38-) fractions from the tissue sources shown. (e, f) Early onset of T cell commitment from LMPP and CD24- CLP in 3 week ATOs revealed by (e) early appearance of DP and (f) T cell committed CD34+CD7+ progenitors. Data are representative of two independent experiments.

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