Supplementary Figure 11: Detailed view of the dendritic spine shown in Fig 4f.

(a-g) Single xy-plane images of the boxed region of Fig. 3iv at different z-heights. (a) z=3.05 μm. (b) z=3.15 μm. (c) z=3.28 μm. (d) z=3.33 μm. (e) z=3.38 μm. (f) z=3.42 μm. (g) z=3.55 μm. (h) Upper left shows a single xy-plane image at a z-height of 3.33 μm (same with d); right shows a single yz-plane image reconstructed from the z-stack image; bottom shows a single xz-plane image reconstructed from the z-stack image; dotted lines of upper left show the single x plane and y plane shown in the right and bottom. Scale bar = 500 nm.