Supplementary Figure 13: Two confocal images of 16-fold expanded Brainbow hippocampus.

Two confocal z-stack images of 16-fold expanded mouse hippocampal circuitry with labeled mTFP (blue), mCherry (green), TagBFP (red). mCherry (green) signals and TagBFP (red) signals were amplified by LNA hybridization-based amplification. mTFP (blue) signals were not amplified. (a-g) Single xy-plane images at different z-heights. (h) Snapshot of a 3-D visualization of the z-stack shown in a-g; see Supplementary Video 8. (i-o) Single xy-plane images at different z-heights. (p) Snapshot of a 3-D visualization of the z-stack shown in i-o; see Supplementary Video 9.