Supplementary Figure 14: Large-area tiling epifluorescence image of Emx1-Cre mouse cortex expressing membrane-bound fluorescent proteins (Brainbow AAVs).

TagBFP (green), mCherry (red), and EYFP (blue) were immunostained, expanded 16-fold via hp-iExM. TagBFP (green) and mCherry (red) signals were amplified by LNA hybridization-based signal amplification; EYFP (blue) signals were not amplified.