Supplementary Figure 3: Schematic of antibodies and DNA around a microtubule.
The 5’ acrydites of the DNA oligos are distributed in the purple shaded region (corresponding to the cylinder of Supplementary Fig. 2a). We constructed a detailed schematic showing a possible arrangement of antibodies and DNA around microtubules, building from Supplementary Fig. 2. As shown in Supplementary Fig. 3, the radius of the microtubule is 12.5 nm, as previously measured by transmission electron microscopy (TEM)1. The radius of an immunostained microtubule (stained with conventional primary and secondary antibodies) is 30 nm, as measured by previous TEM imaging1. The 5’ acrydites of the DNA are distributed in a cylinder with an inner radius of 26.7 nm and outer radius of 33.5 nm, as derived in Supplementary Fig. 2e. As can be seen in Supplementary Fig. 3, the DNA-conjugated secondary antibody makes the radius of the microtubule 3.5 nm larger than the microtubule labeled with regular antibodies (outer radius of 30 nm).
