Supplementary Figure 5: iExM secondary antibodies with a smaller effective probe size. | Nature Methods

Supplementary Figure 5: iExM secondary antibodies with a smaller effective probe size.

From: Iterative expansion microscopy

Supplementary Figure 5

See Supplementary Note 4 for associated results text. (a) Schematic of the DNA-conjugated secondary antibody used in this study. (b) Schematic of a regular secondary antibody bearing fluorophores. (c-e), Three possible designs of DNA-conjugated secondary antibodies with a smaller size. (c) A shorter strand of DNA is used. (d) The acrydite (i.e., gel anchoring group) is positioned at the proximal end of the DNA. (e) A single stranded DNA with an acrydite moiety is conjugated to a secondary antibody.

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