Supplementary Figure 6: Off-target analysis in K562 and HEK293 cells coselected with the AsCpf1 crRNA array vector targeting DNMT1.

(a) Schematic of the dual AsCpf1 and U6-driven crRNA array expression vector. (b) Surveyor assays to determine on-target and off-target activity performed on samples reported in Table 1 and Supplementary Table 1. To facilitate the interpretation of the results, the gels for on-target activity presented in Supplementary Figure 5b,f have been reproduced here. Off-target activity at DNMT1-OT1 was assayed using two different sets of primers since it is found within a repetitive element making its detection more challenging. (c) On-target activity at DNMT1 was also determined using the TIDE assay in both co-selected and transiently transfected cells. (d) Surveyor assays to determine on-target and off-target activity in K562 cells transiently transfected with 1μg and 2μg of the vector shown in (a).