Supplementary Figure 10: Endogenous tagging of the NuA4–TIP60 complex in coselected HEK293 cells. | Nature Methods

Supplementary Figure 10: Endogenous tagging of the NuA4–TIP60 complex in coselected HEK293 cells.

From: Marker-free coselection for CRISPR-driven genome editing in human cells

Supplementary Figure 10

(a) Schematic representation of the experiment. (b) Gene tagging scheme. (c) Western blot analysis on whole cell extracts from HEK293 cells to detect the expression of EPC1-tag and EP400-tag proteins. The FLAG M2 antibody was used to detect tagged proteins and the tubulin antibody was used as a loading control.

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