Supplementary Figure 5: Selection for CRISPR–AsCpf1-driven targeted mutagenesis by coediting ATP1A1 via NHEJ.
From: Marker-free coselection for CRISPR-driven genome editing in human cells
(a) Schematic of the dual AsCpf1 and U6-driven crRNA array expression vector (see also Supplementary Figure 12). (b) K562 cells were transfected with 500ng of the vectors shown in (a), treated and assayed for indels as shown in Supplementary Figure 4d. (c) Same as in (a). (d) Same as in (b) but using 1μg of the vector. (e) HEK293 cells were treated and analyzed as in (b) but the TIDE assay was used to determine the frequency of indels. Value for ATP1A1 relates to the co-selection performed with the DNMT1 crRNA. (f) Same as in (e) but using the Surveyor assay.
