Supplementary Figure 11: CIRCLE-seq sites detected by reference-free site discovery algorithm.
From: CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
Percentage of unique cleavage sites that can be found using a reference-independent site discovery algorithm, for CIRCLE-seq experiments performed with sgRNAs targeting non-repetitive sites in HEK293 (red), K562 (green), and U2OS genomic DNA (blue).
