Supplementary Figure 4: Comparison of CIRCLE-seq and covalently closed linear stem-loop strategies for identifying nuclease-induced off-target effects.
From: CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
(a) Scatterplot of read counts for linear stem-loop and circular (CIRCLE-seq) methods for detecting Cas9 nuclease-induced off-target sites for a sgRNA targeted against VEGFA site 1. (b) Venn diagram showing overlap of sites detected by CIRCLE-seq and alternative linear stem-loop method.
