Supplementary Figure 6: Ascl1 and Dlx2 induction generates mature neurons that form functional inhibitory synapses.
From: Generation of pure GABAergic neurons by transcription factor programming
(a) Spontaneous action potentials (APs) recorded from AD-iN cells, as replated on mouse glia at one week post-induction (WPI) and co-cultured for additional 4 (red, left) to 6 (blue, right) weeks (i); Representative traces (left) and average values (means ± SEM, n = 10 cells, right) of voltage-gated Na+ and K+ currents recorded from AD-iN cells at 6 weeks after glia co-culture (ii). (b) Immunoblot analyses of proteins extracted from AD-iN cells co-cultured with mouse glia cells or mouse glia only. Proteins are identified on the left (b-actin, GFAP, MAP2, Gephyrin, VELI, Munc18, Cask and SYT1 (Synaptotagmin-1). (c) Representative image showing vGAT and Synapsin 1 (SYN1) expression in AD-iN cells co-cultured with mouse glia. (d) Representative traces of sIPSCs (blue trace, i) and extracellular stimulation-induced evoked IPSCs (blue trace, ii), as recorded from AD-iN cells 7 WPI, and were subsequently blocked by 50 μM picrotoxin (red traces, i and ii), a pharmacological blocker of GABAARs.
