Supplementary Figure 8: Long term stability of the GABAergic fate and continuous maturation of AD-iN cells in vivo.
From: Generation of pure GABAergic neurons by transcription factor programming
(a,b) Representative image of an AD-iN cell graft in the mouse cortex 2 weeks post transplantation (w.p.t.). Transplanted cells were detected with EGFP (green) and human nuclei antibodies (blue) and expressed high levels of DCX (magenta). (b) Individual human DCX-positive cells show migrating morphologies. (c-e) After 3 months post transplantation (m.p.t.) we observed robust GABA and NeuN staining in the grafted cells. We also detected an increased number of NeuN+ cells over time.
