Supplementary Figure 10: Analysis of different transcription factor combinations confirmed that Ascl1 and Dlx2 are sufficient to generate SST-, CALB1(CB)-, CALB2(CR)-, VIP- and RELN-expressing cells.
From: Generation of pure GABAergic neurons by transcription factor programming
qRT-PCR analysis was performed for indicated genes using AD-iN cells or iN cells generated by AD plus indicated factors. Expression levels (expressed as Ct values) are color coded as shown. In general, all the “AD plus 1” conditions generated GABAergic neuron populations similar to those induced by AD or AD with Myt1l. All conditions generated iN cells that robustly expressed SST, CALB1, SYN1 and vGAT. Whereas the expression levels of PVAL and NPY are less prominent and more variable.
