Supplementary Figure 4: Detection of GABAergic neuron subtype markers in 5 WPI AD-iN cells. | Nature Methods

Supplementary Figure 4: Detection of GABAergic neuron subtype markers in 5 WPI AD-iN cells.

From: Generation of pure GABAergic neurons by transcription factor programming

Supplementary Figure 4

(a) The robust generation of GABAergic neurons expressing CR, CB and SST from different human ES and iPS cells using Ascl1 and Dlx2. (b) ISL1 was observed in ~ 20% of the AD-iN cells and specific staining of NR2F2 in a small fraction of AD-iN cells (<2%) was detected (c). The bar graph shows the percentage of MAP2 positive cells that also express ISL1. Scale bar: 50 μm.

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