Supplementary Figure 5: Notch inhibitor enhanced the morphologic complexity of AD-iN cells.
From: Generation of pure GABAergic neurons by transcription factor programming
(a) Schematic diagram represents experimental strategy for treating and characterizing cellular phenotype resulting from DAPT treatment in human neurons. (b-c) Brief DAPT treatment (3 or 4 days) at early stage during neuronal induction did not exhibit any effect on the neuronal morphology of AD induced neuronal cells revealed by MAP2 immunofluorescence. However, the mean process length of iN cells significantly increased from day 4 to day 5 after transgene induction independent of DAPT treatment. (d-g) Whereas brief DAPT treatment during day 2-7 after transgene induction was sufficient to improve the complexity of the neuron morphology in terms of the max process length and the total neurite outgrowth (e, g), continuous DAPT treatment from day 2 onwards was necessary to further increase the number of branches (d).
