Supplementary Figure 9: BEV2 structure determination on the basis of XFEL data by molecular replacement using FMDV A22 as a starting model.

(a) Stereo diagram showing the structural differences between BEV2 and FMDV A22 protomers. VP1, VP2, VP3 and VP4 of BEV2 are shown in blue, green, red and yellow respectively, FMDV A22 in grey. (b & c) Stereo diagram showing the electron density maps for the north wall of the canyon which surrounds the icosahedral 5-fold axes (note that FMDVs do not have such a canyon); (b) shows 5-fold real space averaged maps, (c) shows the map after cyclic real space averaging and solvent flattening. The initial map was calculated using phases derived from the FMDV A22 model at 2.3 Å resolution. The final refined structure of BEV2 is in orange and FMDV A22 in cyan.