Supplementary Figure 10: Dye-based AO improves 2P-ISIM imaging in somites (b-e) and neural tube (g-h) of live embryonic zebrafish at 30-32 hpf.
From: Adaptive optics improves multiphoton super-resolution imaging
a) Schematic of embryo, indicating imaging region (higher magnification view). Selected imaging fields at indicated axial depth are shown, before (b, d, g) and after (c, e, h) AO correction and subsequent deconvolution. Comparative wavefronts are also shown as inset. f) Indicates spectral bandwidth used for AO correction with CellTracker Orange and 2P-ISIM imaging. See also Fig. 3. Scale bar: 10 μm.
