Supplementary Figure 11: Dye-based AO improves 2P-ISIM imaging of gastrocnemius muscle of excised mouse leg.
From: Adaptive optics improves multiphoton super-resolution imaging
Rhodamine phalloidin was used to stain actin fibers within the muscle leg and CellTracker Green was added for AO correction. a) Graph indicates spectral region used for AO correction vs. imaging. Selected image slices through two gastrocnemius muscle fibers of differing contractility before (b, e, h, k) and after (c, f, i, l) AO and deconvolution. Normalized line profiles (40 pixels in width) corresponding to red/black lines indicated in each depth are shown in (d, g, j). Higher magnification views of dashed yellow rectangular regions are shown, as are wavefronts (insets). Scale bars: 5 μm for zoomed-out panels, 2 μm for higher magnification views.
