Supplementary Figure 4: Determining optimal sequencing coverage and basal fragment threshold.
From: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
(a) Only PPIs (protein-protein interactions) with 3 or more fragments in the 20 million read depth dataset showed the expected 4-fold or greater increase in coverage in the 80 million read depth dataset, while PPIs with only one or two fragments in the 20 million read depth dataset were frequently not even detected (orange) at the 80 million read depth. (b) At 80 million read depth, the majority of new PPIs that were not detected at the 20 million read depth had less than 10 fragments, with over 90% of PPIs showing less than 3 fragments.
