Supplementary Figure 6: Homodimers do not yield AD-DB PCR products from Cre-recombined plasmids. | Nature Methods

Supplementary Figure 6: Homodimers do not yield AD-DB PCR products from Cre-recombined plasmids.

From: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping

Supplementary Figure 6

(a) Test DB-ORFs and AD-ORFs screened in CRY8930 and Y8800, respectively, using standard 1x1 yeast two-hybrid. (b) Yeast two-hybrid positive spots grown on selection media (1xSC –Leu/-Trp/-His + 1mM 3-AT) that correspond to ORF pairs in a. (c) Colony PCR of yeast two-hybrid positive spots using AD and DB primers to detect the presence of Cre-recombined plasmids. (d) Model of an AD and DB primer-PCR amplicon of a homodimer, that contains on average a 1.66 kilobase pair inverted repeat. Blue boxes indicate homodimer pairs tested.

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