Supplementary Figure 8: Results from 1x1 yeast two-hybrid retest of AtTFIN-1 interactions detected in CrY2H-seq screens. | Nature Methods

Supplementary Figure 8: Results from 1x1 yeast two-hybrid retest of AtTFIN-1 interactions detected in CrY2H-seq screens.

From: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping

Supplementary Figure 8

(a) Representative scoring of retested interactions. (b) Fraction of AtTFIN-1 interactions positive in 1x1 matrix style Y2H retest screen (retest rate) as a function of normalized protein interaction fragments (NPIFs). Novel PPI bin sizes: 59 (1-2.5), 77 (2.5-2.8), 62 (2.8-3), 78 (3-3.2), 64 (3.2-3.4), 84 (3.4-3.7), 82 (3.7-4.1), 74 (> 4.1). Known + novel PPI bin sizes: 65 (1-2.5), 83 (2.5-2.8), 71 (2.8-3), 91 (3-3.2), 71 (3.2-3.4), 99 (3.4-3.7), 92 (3.7-4.1), 84 (> 4.1).

Back to article page