Supplementary Figure 14: CrY2H-seq shows reduced screening costs and time compared to traditional Y2H and BFG-Y2H methods.

(a) Screening cost estimates for two replicate screens of 1000 × 1000 proteins. (b) Screening cost estimates for one screen of 30,000 × 30,000 proteins. Costs for sequencing of Y2H positive clones have been omitted here because we were unable to find a report of the number of reads required per screen size for traditional Y2H or for BFG-Y2H. (c) Screening time estimates. It should be noted that these cost estimates do not take into account preliminary cloning steps as any cloning strategy could be applied to move ORFs into expression plasmids. It should also be noted that this cost comparison is conservative as the cost difference would be larger from traditional Y2H had labor and pipetting costs been accounted for.