Supplementary Figure 15: Determining bait and prey orientation of interacting proteins.
From: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
(a) Bait and prey orientations can be derived from fragments mapping to interactions that contain at least 15 base pairs of Lox77 sequence. Blue and green squares outline the lox sequence parts that reveal prey and bait identity, respectively. The black line exemplifies an orientation-revealing fragment. Amplicon, example fragment, and reads are not drawn to scale; read 1 and read 2 are each a total of 100bp. (b) Overview of fragments mapping to protein-protein interactions (PPIs).
