Supplementary Figure 2: Comparing diploid strains Y8930/Y8800 and CRY8930/Y8800 using a range of interactions.
From: CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
(a) Plate locations of interactions screened in standard 1x1 yeast two-hybrid. Green boxes indicate expected positive interactions. CRY signifies the strain CRY8930/Y8800. Protein names Z53, Z63, ASK1, ZTL, ASK2, and PP2 correspond to the genes AT3G62420, AT5G28770, AT1G75950, AT5G57360, AT5G42190, and AT3G61060, respectively. (b) Diploid culture concentrations reported as OD600/mL. (c) Pictures of diploids after three days of selection on either 1x SC –Leu/-Trp (diploid selection) or 1x SC –Leu/-Trp/-His + 1mM 3-AT (interaction selection); (−) indicates Y8930/Y8800 diploids and (+) indicates CRY8930/Y8800 diploids.
