Supplementary Figure 5: Distributions of bout kinematics with and without motion cancellation for two behavioral contexts.

The positions of zebrafish larvae were recorded by a NIR camera during free swimming behavior in either a stationary behavior chamber (blue and black), or in the tracking microscope with motion cancellation enabled and fluorescent excitation light off (magenta and grey) or on (green). Fish were monitored in two behavioral contexts: spontaneous behavior in the absence of paramecia (black and grey) and prey capture in the presence of paramecia (blue, magenta, and green). For each swim bout, twelve kinematic parameters were calculated (Methods). Each panel shows the distribution of one kinematic parameter. n = 11 fish, 19,250 bouts (magenta, prey capture with tracking enabled), n = 2 fish, 2,826 bouts (green prey capture with tracking enabled and fluorescent excitation light on), n = 9 fish, 14,743 bouts (gray, spontaneous swimming with tracking enabled), n = 16 fish, 49,223 bouts (blue, prey capture with stationary chamber), n = 7 fish, 11,109 bouts (black spontaneous swimming with stationary chamber). All data were collected from awake and freely swimming 6-7 dpf larval zebrafish.