Supplementary Figure 6: Motion cancellation and DIFF imaging do not affect prey capture rate.

Larvae were placed in a behavioral chamber containing 20-30 paramecia with tracking ON or OFF, and with the fluorescence excitation light ON or OFF. A box plot of prey capture rate is shown for all three conditions (Methods). Differences between conditions were not statistically significant (p = 0.570 for tracking ON and fluorescence excitation OFF, p = 0. 0.398 for tracking ON and fluorescence excitation ON, Mann-Whitney U test). n = 16 larvae (tracking OFF), n = 7 larvae (tracking ON with fluorescence excitation OFF), and n = 5 larvae (tracking ON with fluorescence excitation ON). Red horizontal line indicates the median, black box spans the first and third quartiles, and whiskers extend to a maximum of 1.5 × IQR beyond the box. Individual data points are shown in gray. All data were collected from awake and freely swimming 6-7 dpf larval zebrafish.