Supplementary Figure 7: Effects of binning and averaging on shot noise and resolution of DIFF optically sectioned images.
From: Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish
Top, optical sections of the brain using 1 × 1 binning. Bottom, optical sections of the brain using 2 × 2 binning. Left to right, images obtained by averaging increasing numbers of frames (1×, 5×, 10×, and 20×). An ROI of the brain is shown below each whole brain section (yellow box, position of the ROI in brain). Scale bar: 50 μm for whole brain sections and 20 μm for ROIs. To avoid activity-related changes in fluorescence, image data was collected from an anaesthetized 5 dpf elavl3:GCaMP6s larval zebrafish.
