Supplementary Figure 9: Effect of tissue scattering on axial and lateral resolution of DIFF microscopy.

(a) 1 μm fluorescent beads were injected (Methods) into the brain of a 4 dpf nacre^−/− fish. The larva was anaesthetized and imaged at 5 dpf. Beads were distributed throughout the fish brain. The outline of the brain is shown in yellow. Top, dorsal view of the brain. Bottom, sagittal view of the brain. Left, images were acquired with high gain to facilitate visualization of the bead locations within the outline of the fish brain. Right, images were acquired with lower gain to accurately measure bead PSFs without saturated pixels. Measured lateral (b) and axial (c) FWHM of 65 beads as a function of each bead’s axial location within the brain.