Supplementary Figure 12: Neuronal responses to heat pulses in neighboring cells in an elavl3:GCaMP6s fish.

(a) Mean activity (ΔF/F) of an elavl3:GCaMP6s fish (Fig. 4) to heat pulses (5 s duration, 30 s interval). ROI 1 (yellow dot) corresponds to ROI 7 from Fig. 4. ROIs 2-8 (cyan dots) are non-responsive neighboring cells. Scale bars: 100 μm (left) and 20 μm (right). (b) Single trial activity (ΔF/F) of selected neurons in response to individual heat pulses (red lines). (c) Event-triggered average activity (ΔF/F, mean ± s.e.m. are shown in black and gray, n = 18 heat pulses) of the same neurons aligned to the onset of heat (red line). (d) Fish speed (mm/s) across the heat pulses (red lines) from (b). (e) Zoom of the fish speed from (d) across a single trial. (f) Event-triggered average speed (mm/s, mean ± s.e.m. are shown in black and gray, n = 18 heat pulses) of the same fish aligned to the onset of heat (red line).