Supplementary Figure 13: Calcium dynamics of pre-motor and heat-responsive neurons.

Calcium responses to right turns or heat pulses (5 s duration, 30 s interval) are shown for two neurons. Left, event-triggered average activity (ΔF/F, mean ± s.e.m. are shown in black and gray, n = 49 turns) of a hindbrain neuron (top) and habenula neuron (bottom) aligned to right turns (dotted line). Right, event-triggered average activity (ΔF/F, mean ± s.e.m. are shown in black and gray, n = 18 heat pulses) of the same hindbrain neuron (top) and habenula neuron (bottom) aligned to onset of heat (red). Baseline fluorescence (F) is defined as mean fluorescence during a 5 s interval prior to event onset (-10 s to -6 s for activity aligned to turns, -6 s to -1 s for activity aligned to heat onset). All data were collected from an awake and freely swimming 6 dpf elavl3:GCaMP6s larval zebrafish.