Supplementary Figure 9: SLAMseq robustly identifies s4U incorporation events in poly-adenylated RNA.
From: Thiol-linked alkylation of RNA to assess expression dynamics
4-thiouridine incorporation events in total RNA and polyA+ enriched RNA prepared from mESCs subjected to s4U labeling for 6 h was determined by LC-MS/MS (black) and compared to T>C conversions determined by SLAMseq mRNA 3′ end sequencing (red). Based on this analysis, SLAMseq recovers ~90 % of s4U incorporation events in polyadenylated RNA. Data represent mean (center line) ± SD (whiskers) of two independent cell cultures and two independent measurements (LC-MS/MS) or three independent cell cultures (Sequencing).
